Taxon-specific PCR primers to detect two inconspicuous arbuscular mycorrhizal fungi from temperate agricultural grassland

Taxon-specific polymerase chain reaction (PCR) primers enable detection of arbuscular mycorrhizal fungi (AMF, Glomeromycota) in plant roots where the fungi lack discriminative morphological and biochemical characters. We designed and validated pairs of new PCR primers targeted to the flanking regions of the variable domain 1 of the nuclear ribosomal large subunit RNA gene to specifically detect Acaulospora paulinae and an undescribed member of the Diversisporaceae. These two fungal taxa, sporulating late in soil-trap cultures and showing small, faintly coloured spores and weakly staining intraradical structures, were frequently found in roots of Trifolium repens from a high-input agricultural grassland. The newly developed PCR primers may thus enable studies on two inconspicuous AMF taxa that appear to have been overlooked in previous molecular AMF community analyses and for which no specific PCR primers have been publishe

Enabling technologies for audio augmented reality systems

Audio augmented reality (AAR) refers to technology that embeds computer-generated auditory content into a user's real acoustic environment. An AAR system has specific requirements that set it apart from regular human--computer interfaces: an audio playback system to allow the simultaneous perception of real and virtual sounds; motion tracking to enable interactivity and location-awareness; the design and implementation of auditory display to deliver AAR content; and spatial rendering to display spatialised AAR content. This thesis presents a series of studies on enabling technologies to meet these requirements. A binaural headset with integrated microphones is assumed as the audio playback system, as it allows mobility and precise control over the ear input signals. Here, user position and orientation tracking methods are proposed that rely on speech signals recorded at the binaural headset microphones. To evaluate the proposed methods, the head orientations and positions of three conferees engaged in a discussion were tracked. The binaural microphones improved tracking performance substantially. The proposed methods are applicable to acoustic tracking with other forms of user-worn microphones. Results from a listening test investigating the effect of auditory display parameters on user performance are reported. The parameters studied were derived from the design choices to be made when implementing auditory display. The results indicate that users are able to detect a sound sample among distractors and estimate sample numerosity accurately with both speech and non-speech audio, if the samples are presented with adequate temporal separation. Whether or not samples were separated spatially had no effect on user performance. However, with spatially separated samples, users were able to detect a sample among distractors and simultaneously localise it. The results of this study are applicable to a variety of AAR applications that require conveying sample presence or numerosity. Spatial rendering is commonly implemented by convolving virtual sounds with head-related transfer functions (HRTFs). Here, a framework is proposed that interpolates HRTFs measured at arbitrary directions and distances. The framework employs Delaunay triangulation to group HRTFs into subsets suitable for interpolation and barycentric coordinates as interpolation weights. The proposed interpolation framework allows the realtime rendering of virtual sources in the near-field via HRTFs measured at various distances

Soil and plant factors driving the community of soil-borne microorganisms across chronosequences of secondary succession of chalk grasslands with a neutral pH

Although soil pH has been shown to be an important factor driving microbial communities, relatively little is known about the other potentially important factors that shape soil-borne microbial community structure. This study examined plant and microbial communities across a series of neutral pH fields (pH=7.0-7.5) representing a chronosequence of secondary succession after former arable fields were taken out of production. These fields ranged from 17 to >66 years since the time of abandonment, and an adjacent arable field was included as a reference. Hierarchical clustering analysis, nonmetric multidimensional scaling and analysis of similarity of 52 different plant species showed that the plant community composition was significantly different in the different chronosequences, and that plant species richness and diversity increased with time since abandonment. The microbial community structure, as analyzed by phylogenetic microarrays (PhyloChips), was significantly different in arable field and the early succession stage, but no distinct microbial communities were observed for the intermediate and the late succession stages. The most determinant factors in shaping the soil-borne microbial communities were phosphorous and NH4+. Plant community composition and diversity did not have a significant effect on the belowground microbial community structure or diversit

INTERAURAL TIME DELAY PERSONALISATION USING INCOMPLETE HEAD SCANS

ABSTRACT When using a set of generic head-related transfer functions (HRTFs) for spatial sound rendering, personalisation can be considered to minimise localisation errors. This typically involves tuning the characteristics of the HRTFs or a parametric model according to the listener's anthropometry. However, measuring anthropometric features directly remains a challenge in practical applications, and the mapping between anthropometric and acoustic features is an open research problem. Here we propose matching a face template to a listener's head scan or depth image to extract anthropometric information. The deformation of the template is used to personalise the interaural time differences (ITDs) of a generic HRTF set. The proposed method is shown to outperform reference methods when used with high-resolution 3-D scans. Experiments with single-frame depth images indicate that the method is applicable to lower resolution or partial scans which are quicker and easier to obtain than full 3-D scans. These results suggest that the proposed method may be a viable option for ITD personalisation in practical applications

ICASSP 2023 Acoustic Echo Cancellation Challenge

The ICASSP 2023 Acoustic Echo Cancellation Challenge is intended to stimulate research in acoustic echo cancellation (AEC), which is an important area of speech enhancement and is still a top issue in audio communication. This is the fourth AEC challenge and it is enhanced by adding a second track for personalized acoustic echo cancellation, reducing the algorithmic + buffering latency to 20ms, as well as including a full-band version of AECMOS. We open source two large datasets to train AEC models under both single talk and double talk scenarios. These datasets consist of recordings from more than 10,000 real audio devices and human speakers in real environments, as well as a synthetic dataset. We open source an online subjective test framework and provide an objective metric for researchers to quickly test their results. The winners of this challenge were selected based on the average mean opinion score (MOS) achieved across all scenarios and the word accuracy (WAcc) rate.Comment: arXiv admin note: substantial text overlap with arXiv:2202.13290, arXiv:2009.0497